Our basic strategy can be divided into 3 parts: Cloning, Production, Purification
Cloning: Genes encoding the proteins of interest are PCR amplified and cloned into the expression vector based on the requirements of specific expression system. Every cloned gene is sequence verified prior protein production.
Protein production: Our laboratory is fully equipped for the production of recombinant proteins in either prokaryotic (Escherichia coli) or eukaryotic (yeast, insect cells) expression system. The choice of correct expression system depends mostly on the nature of the protein and is done individually for each protein. Target protein production is optimized in small scale and the level of protein production and integrity is verified via SDS-PAGE and/or Western blotting. Optimized conditions are applied for large scale production using modern refrigerated shakers Innova® S44i.
Protein purification: Recombinant proteins are purified using 2 new chromatography systems ÄKTA pure 25M2 (for more details click here) via various column chromatography procedures such as Affinity Chromatography, Anion Exchange Chromatography, Size Exclusion Chromatography etc.