People

Group leader

Roman Tuma
principal investigator
Virus assembly and molecular machines
HPLC and solution scattering techniques
rtuma at prf.jcu.cz
(+420)-38-777-6235
C 01 026
Part time also at University of Leeds, U.K.

Research interests

1. Virus assembly – dsRNA viruses/Avian reovirus, viral factories/viroplasms

2. Mechano-chemical coupling in molecular motors that translocate along nucleic acids and polypeptides e.g. viral ATPases and SecYEG/SecA

3. Assembly of bacteriochlorophylls and structure of light harvesting antennae – chlorosomes

4. Single molecule fluorescence and optical imaging techniques 

We apply combination of advanced biophysical and structural techniques to study self-assembly and function of large macromolecular complexes such as viruses, Sec translocon, helicases and light harvesting complexes. Key biological processes that are studied in our group: RNA packaging and segment assortment in dsRNA viruses of the Reoviridae family; mechanism and dynamics of hexameric helicases and SecA driven polypeptide translocation across membranes. We also use self-assembly of bacterial photosynthetic pigments to create hybrid quantum dot nanostructures for applications in synthetic biology and nanophotonics.

Project manager

Renata Krinesová
project manager
rkrinesova at prf.jcu.cz
(+420)-38-777-5576
Project Department, ÚMBR BC AV, 3rd floor  


Researchers

Joel Crossley

single-molecule fluorescence
joelcrossley at hotmail.com
(+420)-38-777-6238
C 01 038

Filip Dyčka

Reasearch interests: proteomics, MS
Facility responsibility: Mass Spectrometry
fdycka at prf.jcu.cz
(+420)-38-777-6242
C 01 044

Tomáš Fessl
single-molecule fluorescence
fessl at prf.jcu.cz
(+420)-38-777-6238
C 01 038



Zdeněk Franta
molecular biology
zfranta at prf.jcu.cz
(+420)-38-777-6230
C 01 019

Zdeno Gardian
Cryo-EM
gardian at prf.jcu.cz
(+420)-38-777-5459
Laboratory of EM
Part time also at Institute of parasitology BC, ASCR
David Kaftan
Research interests: photosynthesis, spectroscopy, AFM
Facility responsibility: AFM
dkaftan at prf.jcu.cz
(+420)-38-777-6227
C 01 016
Part time also at Institute of Microbiology ASCR
Peter Konik
mass spectrometry
konik at prf.jcu.cz
(+420)-38-777-6242
C 01 044



Ivana Kutá-Smatanová
Research interests: protein crystallogenesis and crystallography
Facility responsibility: X-ray
ivanaks at seznam.cz (kuta at prf.jcu.cz)
C 01 025

David Řeha
computational chemistry
reha at nh.cas.cz
(+420)-38-777-6231
C 01 020
Part time also at the Institute of Microbiology ASCR, Nove Hrady


Post Docs


Paulina Duhita Anindita
Molecular Biology and protein crystallography
anindita.duhita at gmail.com
(+420)-38-777-6230
C 01 019





Pavel Grinkevich
Protein crystalloraphy and molecular biology
pavel.grinkevich at gmail.com
(+420)-38-777-6235
C 01 026




PhD students

Barbora Kaščáková
Mol-bio and structural studies of sigmaNS and P4 proteins
Supervisor: doc. RNDr. Ivana Kutá-Smatanová, Ph.D.
barbora.karrafova at gmail.com
C 01 024

Eva Ďurinová

Cryo Electron Microscopy in Structural Studies of Biological Specimen.
Supervisor: Zdeno Gardian
evadurinova12 at gmail.com
Laboratory of EM

 

Petra Havlíčková

Structural studies of TBEV NS proteins.
Supervisor: doc. RNDr. Ivana Kutá-Smatanová, Ph.D.
havlickova.pet at seznam.cz
C 01 024



Štěpánka Tomková
The role of TBEV nonstructural proteins on the virus replication
Supervisor: Zdeněk Franta
tomkos00 at prf.jcu.cz
C 01 019




Lab Technician

Zuzana Světelová

Lab technician
svetez00 at prf.jcu.cz
(+420)-38-777-6230
C 01 018



MSc Students

Eliška Kotounová

BSc Students


Marco Halbeisen
Jacob Schmelzer