People

Group leader

Roman Tuma
principal investigator
Virus assembly and molecular machines
HPLC and solution scattering techniques
rtuma at prf.jcu.cz
(+420)-38-777-6235
C 01 026
Part time also at University of Leeds, U.K.

Research interests

1. Virus assembly – dsRNA viruses/Avian reovirus, viral factories/viroplasms

2. Mechano-chemical coupling in molecular motors that translocate along nucleic acids and polypeptides e.g. viral ATPases and SecYEG/SecA

3. Assembly of bacteriochlorophylls and structure of light harvesting antennae – chlorosomes

4. Single molecule fluorescence and optical imaging techniques 

We apply combination of advanced biophysical and structural techniques to study self-assembly and function of large macromolecular complexes such as viruses, Sec translocon, helicases and light harvesting complexes. Key biological processes that are studied in our group: RNA packaging and segment assortment in dsRNA viruses of the Reoviridae family; mechanism and dynamics of hexameric helicases and SecA driven polypeptide translocation across membranes. We also use self-assembly of bacterial photosynthetic pigments to create hybrid quantum dot nanostructures for applications in synthetic biology and nanophotonics.

Project manager

Renata Krinesová
project manager
rkrinesova at prf.jcu.cz
(+420)-38-777-5576
Project Department, ÚMBR BC AV, 3rd floor  


Researchers

Joel Crossley

single-molecule fluorescence
joelcrossley at hotmail.com
(+420)-38-777-6238
C 01 038

Filip Dyčka

Reasearch interests: proteomics, MS
Facility responsibility: Mass Spectrometry
fdycka at prf.jcu.cz
(+420)-38-777-6242
C 01 044

Tomáš Fessl
single-molecule fluorescence
fessl at prf.jcu.cz
(+420)-38-777-6238
C 01 038



Zdeněk Franta
molecular biology
zfranta at prf.jcu.cz
(+420)-38-777-6230
C 01 019

Zdeno Gardian
Cryo-EM
gardian at prf.jcu.cz
(+420)-38-777-5459
Laboratory of EM
Part time also at Institute of parasitology BC, ASCR
David Kaftan
Research interests: photosynthesis, spectroscopy, AFM
Facility responsibility: AFM
dkaftan at prf.jcu.cz
(+420)-38-777-6230
C 01 019
Part time also at Institute of Microbiology ASCR
Peter Konik
mass spectrometry
konik at prf.jcu.cz
(+420)-38-777-6242
C 01 044



Ivana Kutá-Smatanová
Research interests: protein crystallogenesis and crystallography
Facility responsibility: X-ray
ivanaks at seznam.cz (kuta at prf.jcu.cz)
C 01 025

David Řeha
computational chemistry
reha at nh.cas.cz
(+420)-38-777-6231
C 01 020
Part time also at the Institute of Microbiology ASCR, Nove Hrady

Lab Technician

Zuzana Světelová

Lab technician
svetez00 at prf.jcu.cz
(+420)-38-777-6230
C 01 018

PhD students

Barbora Kaščáková
Mol-bio and structural studies of sigmaNS and P4 proteins
Supervisor: doc. RNDr. Ivana Kutá-Smatanová, Ph.D.
barbora.karrafova at gmail.com
C 01 024


Eva Ďurinová

Cryo Electron Microscopy in Structural Studies of Biological Specimen.
Supervisor: Zdeno Gardian
Laboratory of EM



MSc and BSc Students

Kateřina Vejvodová
Volodymyr Tsibulsky
Eliška Kotounová
Matin Kazemi
Andreea-Adriana Avram
Marc Ninou-Codina
Siroun Moura
Farah Khalil
Nelli Manoczki